Publications

Wang Lab Publications

2020

116. Demet Candas-Green, Bowen Xie, Jie Huang, Ming Fan, Aijun Wang, Cheikh Menaa, Yanhong Zhang, Lu Zhang, Di Jing, Soheila Azghadi, Weibing Zhou, Lin Liu, Nian Jiang, Tao Li, Tianyi Gao, Colleen Sweeney, Rulong Shen, Tzu-yin Lin, Chong-xian Pan, Omer M. Ozpiskin, Gayle Woloschak, David J. Grdina, Andrew T. Vaughan, Ji Ming Wang, Shuli Xia, Arta M. Monjazeb, William J. Murphy, Lun-Quan Sun, Hong-Wu Chen, Kit S. Lam, Ralph R. Weichselbaum, Jian Jian Li. Dual blockade of CD47 and HER2 eliminates radioresistant breast cancer cells. Nature Communications. 2020, Sept 14;11(1): 4591. PMID: 32929084. DOI: 10.1038/s41467-020-18245-7

2020 Nature Comm Jian Jian Li
A cellular model of radioresistant breast cancer is proposed in which two cell surface receptors CD47 and HER2 coordinately contribute to the aggressive phenotype of breast cancer cells surviving radiotherapy
The radioresistant breast cancer cells that are enriched with breast cancer stem cells can not only survive radiation but also enhance their immune-escaping ability via CD47-mediated anti-phagocytosis. CD47 transcription is regulated via HER2–NF-κB axis leading to the enhancement of both receptors on the cell surface. Thus, HER2-promoted intrinsic proliferation is synergized with the CD47-initiated extrinsic anti-phagocytosis; a dual blockade of these two receptors may eliminate the radioresistant breast cancer cells in breast cancer radiotherapy.
 

116. Dake Hao, Ruiwu Liu, Kewa Gao, Chuanchao He, Siqi He, Cunyi Zhao, Gang Sun, Diana L Farmer, Alyssa Panitch, Kit S Lam, Aijun Wang. Developing an injectable nanofibrous extracellular matrix hydrogel with an integrin αvβ3 ligand to improve endothelial cell survival, engraftment and vascularization. Frontiers in Bioengineering and Biotechnology. 2020, Jul 29;8:890. July 29. PMID: 32850742. PMCID: PMC7403189. DOI: 10.3389/fbioe.2020.00890

LXW7 collagen hydrogel EC survival
ECFC engraftment in LXW7-modified collagen hydrogel after implantation
(A) IVIS imaging of implanted ECFC engraftment in untreated collagen hydrogel (right side on animal) and LXW7-modified collagen hydrogel (left side on animal) at different time points. The belly of animal was facing you in the image. (B) Quantification of the luciferase intensity of the engrafted ECFCs in collagen hydrogel with or without LXW7 modification at different time points. Data were expressed as mean ± standard deviation: ∗p < 0.05 (n = 4).
 

115. Rogério Martins Amorim, Kaitlin C. Clark, Naomi J. Walker, Priyadarsini Kumar, Kyle Herout, Dori L. Borjesson, Aijun Wang. Placenta-Derived Multipotent Mesenchymal Stromal/Stem Cells: A Promising Potential Cell-Based Therapy for Canine Inflammatory Brain Disease. Stem Cell Research & Therapy. 2020, Jul 22;11(1):304. PMID: 32698861. DOI: 10.1186/s13287-020-01799-0

ASC vs PMSC
Canine ASCs and PMSCs inhibit lymphocyte proliferation in a contact-dependent manner
Canine adipose-derived MSCs (ASCs) and placenta-derived MSCs (PMSCs) possess immune-suppressive functions. a Canine ASCs and PMSCs co-incubated with stimulated peripheral blood mononuclear cells (PBMCs) suppress lymphocyte proliferation. PMSCs significantly decrease lymphocyte proliferation more potently as compared to ASCs. Transwells were added to remove physical contact between MSCs and mitogen (ConA) stimulated PBMCs. Lymphocyte proliferation increased when ASCs and PMSCs were not in direct contact with activated PBMCs. b To determine the role of indoleamine 2,3 dioxygenase (IDO) and prostaglandin E2 (PGE2) in MSC-mediated immunosuppression 1-methyl-DL-tryptophan (1-MT) and indomethacin was added to co-cultures to block each respective mediator. Blocking of IDO activity using 1-MT resulted in no alterations in MSC-mediated suppression of PBMC proliferation. Blocking PGE2 using indomethacin resulted in a loss of lymphocyte inhibition by ASCs but no effects were observed by PMSCs. PBMC stimulation is normalized and data is presented as a reduction to each respective donor. 
 

114. Zhengkui Zhang, Xiuwu Fang, Xiaojin Wu, Li Ling, Feng Chu, Jingxian Li, Shuai Wang, Jia Zang, Bo Zhang, Sheng Ye, Long Zhang, Bing Yang, Shixian Lin, Huizhe Huang, Aijun Wang, Fangfang Zhou. Acetylation-Dependent Deubiquitinase OTUD3 Controls MAVS Activation in Innate Antiviral Immunity. Molecular Cell. 2020 Jul 16;79(2):304-319.e7. PMID: 32679077. DOI: 10.1016/j.molcel.2020.06.020

2020 Mol Cell

113. Dake Hao, Hila Shimshi Swindell, Lalithasri Ramasubramanian, Ruiwu Liu, Kit S Lam, Diana L Farmer, Aijun Wang. Extracellular matrix mimicking nanofibrous scaffolds modified with mesenchymal stem cell-derived extracellular vesicles for improved vascularization. Frontiers in Bioengineering and Biotechnology. 2020, June 25;8:633. PMID: 32671037. PMCID: PMC7329993. DOI: 10.3389/fbioe.2020.00633

LLP2A EV attachment
Effect of LLP2A modification on PMSC-EV attachment on electrospun scaffolds 
(A) SEM images of PMSC-EV attachment on untreated electrospun scaffold (a), 50% LLP2A modified electrospun scaffold (b) and 100% LLP2A modified electrospun scaffold (c). Scale bar = 500 nm. (B) Quantification of the numbers of PMSC-EVs on the different modified electrospun scaffolds. Data were expressed as mean ± standard deviation: **p < 0.01 (n = 5).
 

112. Alexandra M. Iavorovschi, Aijun Wang. Engineering Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles with Improved Targeting and Therapeutic Efficiency for the Treatment of Central Nervous System Disorders. Neural Regeneration Research. 2020 Dec; 15(12):2235-2236. PMID: 32594034. DOI: 10.4103/1673-5374.284982

Cell engineering to improve EV production yield, targeting and therapeutic efficiency
Cell engineering to improve EV production yield, targeting and therapeutic efficiency
Cell engineering can be done by through (1) extracellular engineering by using bioreactor, 2D culture with a stressor agent, and biomaterial scaffolds and/or microcarriers; (2) intracellular engineering by genetically engineer the parent cells, optimizing EV biogenesis pathways, and loading exogenous nucleic acids into the parent cell.
 

111. Lili Zhou, Yuqi Zhang, Yongqiang Wang, Meirong Zhang, Wenhuan Sun, Tong Dai, Aijun Wang, Xiaojin Wu, Suping Zhang, Shuai Wang, Fangfang Zhou. A Dual Role of Type I Interferons in Antitumor Immunity. Advanced Biosystems. 2020, March 13. Online ahead of print. https://doi.org/10.1002/adbi.201900237

110. Shujing Guo, Yan Wang, Aijun Wang. Identity and lineage fate of proteolipid protein 1 gene (Plp1)-expressing cells in the embryonic murine spinal cord. Developmental Dynamics. 2020 Apr 30. Online ahead of print. PMID: 32353175. DOI: 10.1002/dvdy.184

109. Sheng Zhang, Bokyung Kim, Xiaoqing Zhu, Xuehong Gui, Yan Wang, Zhaohui Lan, Preeti Prabhu, Kenneth Fond, Aijun Wang, Fuzheng Guo. Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways. Nature Communications. 2020. Apr 24;11(1):2027.  PMID: 32332719. DOI: 10.1038/s41467-020-15656-4.

Nature Communications 2020

 

108. Melanie Rose, Kewa Gao, Elizabeth Cortez-Toledo, Emmanuel Agu, Alicia A. Hyllen, Kelsey Conroy, Guangjin Pan, Jan A. Nolta, Aijun Wang#, Ping Zhou#. Endothelial cells derived from patients' induced pluripotent stem cells for sustained factor VIII delivery and the treatment of hemophilia A. STEM CELLS Transl Med. 2020, Mar 12. #Co-corresponding author. PMID: 32162786. DOI: https://doi.org/10.1002/sctm.19-0261

The study was featured and highlighted by Stem Cells Translational Medicine (SCTM) and many other news outlets, such as SFGate, bioportfolio, etc:

Featured as the front cover (Volume 9, Number 6, June 2020). 

SCTM Front Cover

This study is in the top 5% (the 97th percentile) of all research outputs ever tracked by Altmetric: https://wiley.altmetric.com/details/77402928

Featured by Stem Cells Translational Medicine (SCTM)https://stemcellsportal.com/press-releases/induced-pluripotent-stem-cells-show-success-treating-hemophilia-mice

Medical Health News (Alternative Medicine): http://www.medicalhealthnews.net/cell-btherapyb-seen-to-ease-blood-loss-in-hemophilia-a-mouse-model

Hemophilia News Today: https://hemophilianewstoday.com/2020/03/23/cell-therapy-seen-to-ease-blood-loss-in-hemophilia-a-mouse-model/

Induced Pluripotent Stem Cells Show Success in Treating Hemophilia A in Mice
Induced Pluripotent Stem Cells Show Success in Treating Hemophilia A in Mice

107. Dake Hao, Yahan Fan, Wenwu Xiao, Ruiwu Liu, Christopher Pivetti, Tanaya Walimbe, Fuzheng Guo, Xinke Zhang, Diana L Farmer, Fengshan Wang, Alyssa Panitch, Kit S Lam, Aijun Wang. Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells. Acta Biomaterialia. 2020, May;108:178-193. PMID: 32151698. DOI: 10.1016/j.actbio.2020.03.005

LXW7 modification improved graft patency. New capillary formation was exhibited on untreated grafts (A) and LXW7-modified grafts (B) at week 6 after implantation. Arrows in (B) indicate new capillary formation was present on the LXW7-modified grafts. Representative H&E staining of the cross sections of the vascular grafts at week 6 after implantation showed that the untreated graft had a significant amount of thrombus formation (C) and LXW7-modified graft exhibited a widely open lumen and no thrombus formation (D). (E) Immunostaining of cross sections of LXW7-modified grafts with mature EC marker CD31. (F) The patency of the grafts was examined at various time points. Each group at each time point included 6 animals.
LXW7 modification improved patency of the small-diameter vascular grafts after implantation in the rodent model
New capillary formation was exhibited on untreated grafts (A) and LXW7-modified grafts (B) at week 6 after implantation. Arrows in (B) indicate new capillary formation was present on the LXW7-modified grafts. Representative H&E staining of the cross-sections of the vascular grafts at week 6 after implantation showed that the untreated graft had a significant amount of thrombus formation (C) and LXW7-modified graft exhibited a widely open lumen and no thrombus formation (D). (E) Immunostaining of cross-sections of LXW7-modified grafts with mature EC marker CD31. (F) The patency of the grafts was examined at various time points. Each group at each time point included 6 animals.

106. Ngan F. Huang, Ovijit Chaudhuri, Patrick Cahan, Aijun Wang, Adam J. Engler, Yingxiao Wang, Sanjay Kumar, Ali Khademhosseini, Song Li. Multi-scale cellular engineering: From molecules to organ-on-a-chip. APL Bioengineering. 2020, 4, 010906. DOI: 10.1063/1.5129788

105. Jin-Ho Kang, Janelle Kaneda, Jae-Gon Jang, Kumaresan Sakthiabirami, Elaine Lui, Carolyn Kim, Aijun Wang, Sang-Won Park, Yunzhi Peter Yang. The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering. Micromachines 2020, 11(3), 273. DOI: https://doi.org/10.3390/mi11030273

104. Galganski L.A, Yamashiro K.J, Pivetti C.D, Keller B.A, Becker J.C, Brown E.G, Saadai P, Hirose S, Wang A, Farmer D.L. A Decade of Experience with the Ovine Model of Myelomeningocele: Risk Factors for Fetal Loss. Fetal Diagn Ther. 2020 Feb 25:1-7. PMID: 32097922. DOI: 10.1159/000505400

103. Qiang Ao, Shilin Wang, Qing He, Hirotomo Ten, Kenichi Oyama, Akihiro Ito, Jing He, Rabia Javed, Aijun Wang, Akira Matsuno. Fibrin Glue/Fibronectin/Heparin-Based Delivery System of BMP2 Induces Osteogenesis in MC3T3-E1 Cells and Bone Formation in Rat Calvarial Critical-Sized Defects. ACS Appl. Mater. Interfaces, 2020, Mar 18;12(11):13400-13410. PMID: 32091872. DOI: 10.1021/acsami.0c01371

102. Kewa Gao, Siqi He, Priyadarsini Kumar, Diana Farmer, Jianda Zhou, Aijun Wang. Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration. World J Stem Cells. Feb 26, 2020; 12(2): 123-138. DOI: 10.4252/wjsc.v12.i2.123

Isolation procedure of endothelial colony-forming cells from placental chorionic villi
Isolation procedure of endothelial colony-forming cells from placental chorionic villi.
A: Depiction of the isolation of chorionic villus endothelial colony-forming cells from chorionic-villus cells by enzymatic dissociation of chorionic villus tissue, followed by CD31 magnetic bead sorting and subsequent manual clonal isolation and expansion; B: Morphology of the colonies formed by CD31+-sorted cells. CD31 was expressed only by colonies that had a cobblestone-like morphology and not by ones that displayed spindle-shaped morphology. Two representative colonies are shown for each morphology; C: Immunofluorescence staining of isolated
colonies for expression of CD31 and CD144.

101. Dake Hao, Bowen Ma, Chuanchao He, Ruiwu Liu, Diana L. Farmer, Kit Lam, Aijun Wang. Surface Modification of Polymeric Electrospun Scaffolds via a Potent and High-Affinity Integrin α4β1 Ligand Improved the Adhesion, Spreading and Survival of Human Chorionic Villus-Derived Mesenchymal Stem Cells: A New Insight for Fetal Tissue Engineering. Journal of Materials Chemistry B, 2020 Feb 26;8(8):1649-1659. PMID: 32011618. DOI: 10.1039/c9tb02309g

(A) SEM images of CV-MSC coverage on an untreated scaffold surface (a) and LLP2A-modified scaffold surface (b) for 2 days; (B) Quantification and the correlative statistical analysis of cell-covered area from the SEM images. Data were expressed as mean ± standard deviation: **p < 0.01 (n = 4).
High-Affinity Integrin α4β1 Ligand LLP2A Improved the Adhesion, Spreading and Survival of Human Chorionic Villus-Derived Mesenchymal Stem Cells
(A) SEM images of CV-MSC coverage on an untreated scaffold surface (a) and LLP2A-modified scaffold surface (b) for 2 days; (B) Quantification and the correlative statistical analysis of cell-covered area from the SEM images. Data were expressed as mean ± standard deviation: **p < 0.01 (n = 4).

 

2019

100. Lalithasri Ramasubramanian, Priyadarsini Kumar, Aijun Wang. Engineering Extracellular Vesicles as Nanotherapeutics for Regenerative Medicine. Biomolecules. 2019 Dec 28;10(1). PMID: 31905611. DOI: 10.3390/biom10010048.

2019 Lalitha Biomolecules

EV engineering has emerged in order to augment the natural properties of EVs and to recapitulate their function in semi-synthetic and synthetic EVs

99. Jeffrey J. D. Henry, Lawrence Delrosario, Jun Fang, Sze Yue Wong, Qizhi Fang, Richard Sievers, Surya Kotha, Aijun Wang, Diana Farmer, Praneeth Janaswamy, Randall J. Lee, Song Li. Development of Injectable Amniotic Membrane Matrix for Postmyocardial Infarction Tissue Repair. Advanced Healthcare Materials, 2020 Jan;9(2):e1900544. PMID: 31778043. DOI: 10.1002/adhm.201900544

98. Juanjuan Tan, Yilin Xie, Aihong Yao, Yingchun Qin, Lei Li, Li Shen, Xiaoqin Zhang, Chunfang Xu, Xuesong Jiang, Aijun Wang*, Zhiqiang Yan*. Long Noncoding RNA-dependent Regulation of Vascular Smooth Muscle Cell Proliferation and Migration in Hypertension. The International Journal of Biochemistry & Cell Biology. *Co-Corresponding author. 2020 Jan;118:105653. PMID: 31743794. DOI: 10.1016/j.biocel.2019.105653

97. Kaitlin Clark, Sheng Zhang, Sylvain Barthe, Priyadarsini Kumar, Christopher Pivetti, Nicole Kreutzberg, Camille Reed, Yan Wang, Zachary Paxton, Diana Farmer, Fuzheng Guo, Aijun Wang. Neuroprotective Placental Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Myelin Regeneration in an Animal Model of Multiple Sclerosis. Cells, 20198(12), 1497. PMID: 31771176. DOI: 10.3390/cells8121497

Neuroprotective PMSC-EVs Promote Myelin Regeneration in an Animal Model of Multiple Sclerosis (Experimental design overview)

96. Dake Hao, Chuanchao He, Bowen Ma, Lee Lankford, Lizette Reynaga, Diana L. Farmer, Fuzheng Guo, Aijun Wang. Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering. Stem Cells International. 2019, Nov 12; 2019:9695239. PMID: 31781252. PMCID: PMC6874947. DOI: 10.1155/2019/9695239

2019 Stem cell International
We developed an optimal HP protocol to enhance the survival and proangiogenic capacity of CV-MSCs for improving clinical outcomes in fetal applications.

95. Laura Galganski, Priyadarsini Kumar, Melissa Vanover, Christopher Pivetti, Jamie Anderson, Lee Lankford, Zachary Paxton, Karen Chung, Chelsey Lee, Hegazi MS, Kaeli Yamashiro, Aijun Wang, Diana Farmer. In Utero Treatment of Myelomeningocele with Placental Mesenchymal Stromal Cells Rescues Ambulation in the Ovine Model – Selection of an Optimal Cell Line in Preparation for Clinical Trials. The Journal of Pediatric Surgery. 2019, Oct 21. pii: S0022-3468(19)30681-5. [Epub ahead of print]. PMID: 31672407. DOI: 10.1016/j.jpedsurg.2019.09.029

94. Leora Goldbloom-Helzner, Dake Hao, Aijun Wang. Developing regenerative treatments for developmental defects, injuries, and diseases using extracellular matrix collagen-targeting peptides. International Journal of Molecular Sciences. 2019, 20(17), 4072. PMID: 31438477. DOI: 10.3390/ijms20174072.

93. Ya-Nan Xue, Yu Yan, Zi-Zi Chen, Jia Chen, Feng-Jie Tang, Hui-Qing Xie, Shijie Tang, Ke Cao, Xiao Zhou, Aijun Wang, Jianda Zhou. LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose‐derived stem cells by sponging miR-143. J Cell Biochem. 2019 Jul 1. [Epub ahead of print]. PMID: 31264280. DOI: 10.1002/jcb.29232

92. Can Liu, Wei Chen, Zizi Chen, Yu Yan, Qing Wang, Huiqing Xie, Xiang Chen, Aijun Wang, Shijie Tang, Jianda Zhou. Studies on the treatment of melanoma with folate acid conjugated dextran and lauryl alcohol loaded with IMD0354. Onco Targets Ther. 2019 Jun; 12: 4655–4663.  PMID: 31354298. PMCID: PMC6585409. DOI: 10.2147/OTT.S207685

91. Yiqian Zhu, Xian Li, Randall Raphael R. Janairo, George Kwong, Anchi D. Tsou, Julia S. Chu, Aijun Wang, Song Li. Matrix Stiffness Modulates the Differentiation of Neural Crest Stem Cells In Vitro and In Vivo. Journal of Cellular Physiology. 2019 May; 234(5):7569-7578. PMID: 30368818. DOI: 10.1002/jcp.27518

90. Priyadarsini Kumar, James C. Becker, Kewa Gao, Randy P. Carney, Lee Lankford, Benjamin A. Keller, Kyle Herout, Kit S. Lam, Diana Farmer, Aijun Wang. Neuroprotective Effect of Placenta-derived Mesenchymal Stromal Cells: Role of Exosomes. The FASEB Journal. 2019 May; 33(5):5836-5849. PMID: 30753093. DOI: 10.1096/fj.201800972R

Press release:

https://fasebj.altmetric.com/details/55408320/news

https://www.eurekalert.org/pub_releadses/2019-03/uoc--rat030619.php

https://health.ucdavis.edu/publish/news/newsroom/13630

https://bme.ucdavis.edu/news/faseb-journal-spotlights-uc-davis-researchers-work-stem-cell-based-neurological-therapies

image

Neuroprotective effect of PMSC‐derived exosomes

89. Melissa Vanover, Christopher Pivetti, Laura Galganski, Priyadarsini Kumar, Lee Lankford, Douglas Rowland, Zachary Paxton, Bailey Deal, Aijun Wang, Diana Farmer. Spinal Angulation: A Limitation of the Fetal Lamb Model of Myelomeningocele. Fetal Diagn Ther. 2019 Apr; 10:1-9. PMID: 30970373. DOI: 10.1159/000496201

88. Yong Chen, Ke Cao, Jingjing Li, Aijun Wang, Lichun Sun, Jintian Tang, Wei Xiong, Xiao Zhou, Xiang Chen, Jianda Zhou, Yan Liu. Overexpression of long non-coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR-205-EGLN2 pathway. Cancer Medicine. 2019 Apr; 8(4):1744-1754. PMID: 30843652. DOI: https://doi.org/10.1002/cam4.2046

87. Junlin Liao, Yong Chen, Jia Chen, Bin He, Li Qian, Jiaqin Xu, Aijun Wang, Qingfeng Li, Hongju Xie, Jianda Zhou. Auricle shaping using 3D printing and autologous diced cartilage. Laryngoscope. 2019 Nov; 129(11):2467-2474. PMID: 30843613. PMCID: PMC6850318. DOI: 10.1002/lary.27752

86. Feng Hu, Yu Yan, Chu-wang Wang, Yu Liu, Jing-jing Wang, Fang Zhou, Qing-hai Zeng, Xiao Zhou, Jia Chen, Aijun Wang, Jianda Zhou. Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice. Chinese Journal of Integrative Medicine. 2019 Mar;25(3):203-209. PMID: 30552545. DOI: 10.1007/s11655-018-3060-9

85. Jingjing Li, Jia Chen, Shaohua Wang, Ping Li, Changli Zheng, Xiao Zhou, Yongguang Tao, Xiang Chen, Lichun Sun, Aijun Wang, Ke Cao, Shijie Tang, Jianda Zhou. Blockage of transferred exosome-shuttled miR494 inhibits melanoma growth and metastasis. Journal of Cellular Physiology. 2019 Feb 5. doi: 10.1002/jcp.28234. [Epub ahead of print]. PMID: 30723916. DOI: 10.1002/jcp.28234

84. Connor Long, Lee Lankford, Aijun Wang. Cell-based In Utero Therapies for Spina Bifida: Implications for Neural Regeneration. Neural Regeneration Research. 2019, 14(2):260-261. PMID: 30531007. PMCID: PMC6301160. DOI: 10.4103/1673-5374.244786

83. Kewa Gao, Priyadarsini Kumar, Elizabeth Cortez-Toledo, Dake Hao, Lizette Reynaga, Melanie Rose, Chuwang Wang, Diana Farmer, Jan Nolta, Jianda Zhou, Ping Zhou, Aijun Wang. Potential long-term treatment of hemophilia A by neonatal co-transplantation of cord blood derived endothelial colony-forming cells and placental mesenchymal stromal cells. Stem Cell Research & Therapy. 2019 Jan 22;10(1):34. PMID: 30670078. PMCID: PMC6341603. DOI: 10.1186/s13287-019-1138-8

Press release:

https://hemophilianewstoday.com/2019/01/28/cell-therapy-long-term-treatment-hemophilia/

https://bme.ucdavis.edu/news/uc-davis-researchers-strengthen-stem-cell-transplants-better-patient-outcomes

Long-term monitoring cell engraftment in mice transplanted at neonatal age
Long-term monitoring cell engraftment in mice transplanted at neonatal age
(A) Representative bioluminescence image of NSG mice transplanted with ECFCs only, PMSCs only or ECFCs plus PMSCs at 3–5 days after birth over 26 weeks post-transplantation. (B) Quantitative analysis of the bioluminescence signal intensity in these mice over time. (C) Real-time PCR analysis of different transplantation groups at 26 weeks post-transplantation for the presence of human cells using human-specific ERV3 and mouse GAPDH primers. (D) Comparison of cell retention at 4 to 24 weeks of post-transplantation of mice between transplanted cells at the neonatal period and adult. 

82. Zhenyu Zhou, Hai Jiang, Kangsheng Tu, Wei Yu, Jianlong Zhang, Zhigang Hu, Heyun Zhang, Dake Hao, Pinbo Huang, Jie Wang, Aijun Wang#, Zhiyu Xiao#, Chuanchao He#. ANKHD1 is required for SMYD3 to promote tumor metastasis in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research. 2019 Jan 15;38(1):18. #Co-corresponding author. PMID: 30646949. PMCID: PMC6332640. DOI: https://doi.org/10.1186/s13046-018-1011-0

81. Zhengkui Zhang, Jinjin Du, Shuai Wang, Li Shao, Ke Jin, Fang Li, Bajin Wei, Wei Ding, Peifen Fu, Hansvan Dam, Aijun Wang, Jin Jin, Chen Ding, BingYang, Min Zheng, Xin-Hua Feng, Kun-Liang Guan, Long Zhang. OTUB2 Promotes Cancer Metastasis via Hippo-Independent Activation of YAP and TAZ. Molecular Cell. 2019 Jan 3;73(1):7-21.e7PMID: 30472188. DOI: 10.1016/j.molcel.2018.10.030

80. Melissa Vanover, Christopher Pivetti, Lee Lankford, Priyadarsini Kumar, Laura Galganski, Sandra Kabagambe, Benjamin Keller, James Becker, Y. Julia Chen, Karen Chung, Chelsey Lee, Zachary Paxton, Bailey Deal, Laura Goodman, Jamie Anderson, Guy Jensen, Aijun Wang, Diana Farmer. High Density Placental Mesenchymal Stromal Cells Provide Neuronal Preservation and Improve Motor Function Following In Utero Treatment of Ovine Myelomeningocele. The Journal of Pediatric Surgery. 2019 Jan;54(1):75-79. PMID: 30529115. PMCID: PMC6339576. DOI: https://doi.org/10.1016/j.jpedsurg.2018.10.032

2018

79. Sheng Zhang, Abeer Rasai, Yan Wang, Jie Xu, Peter Bannerman, Daffcar Erol, Danayit Tsegaye, Aijun Wang, Athena Soulika, Xiangjiang Zhan, Fuzheng Guo. The stem cell factor Sox2 is a positive timer of oligodendrocyte development in the postnatal murine spinal cord. Molecular Neurobiology. 2018 Dec; 55(12):9001-9015. PMID: 29623612. PMCID: PMC6173662. DOI: 10.1007/s12035-018-1035-7

78. Jennifer Phan, Priyadarsini Kumar, Dake Hao, Kewa Gao, Diana Farmer, Aijun Wang. Engineering mesenchymal stem cells to improve their exosome efficacy and yield for cell-free therapy. Journal of Extracellular Vesicles. 2018, Sep 26; 7(1):1522236. PMID: 30275938. PMCID: PMC6161586. DOI: 10.1080/20013078.2018.1522236

The different forms of an extracellular matrix and their interactions with the cells
The different forms of an extracellular matrix and their interactions with the cells.
The tissue culture-treated plastic surface is the most common surface for culturing the MSCs (A). The ECM sheet can be in the form of a decellularised native ECM or an artificial matrix, which can include different oriented fibers (B). A hydrogel can be manufactured from the decellularized ECM or a chemical mixture for culturing seeded or encapsulated MSCs (C). The porous scaffold is the most similar to the native environment of the MSCs, perhaps allowing the most suitable cultural environment (D).

77. Qi Wang, Jia Chen, Aijun Wang, Lichun Sun, Li Qian, Xiao Zhou, Yu Liu, Shijie Tang, Xiang Chen, Yan Cheng, Ke Cao, Jianda Zhou. Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion. Oncology Reports. 2018 Apr;39(4):1813-1824. PMID: 29436673. DOI: 10.3892/or.2018.6263

76. Volodymyr Ryzhuk, Xu-xin Zeng, Xijun Wang, Veniamin Melnychuk, Lee Lankford, Diana Farmer, Aijun Wang. Human amnion extracellular matrix derived bioactive hydrogel for cell delivery and tissue engineering. Materials Science & Engineering C. 2018 Apr 1;85: 191-202. PMID: 29407148. DOI: 10.1016/j.msec.2017.12.026

Human amnion extracellular matrix derived bioactive hydrogel for cell delivery and tissue engineering
Human amnion extracellular matrix derived bioactive hydrogel for cell delivery and tissue engineering

75. Sheng Zhang, Xiaoqing Zhu, Xuehong Gui, Christopher Croteau, Lanying Song, Jie Xu, Aijun Wang, Peter Bannermann, Fuzheng Guo. Sox2 is Essential for Oligodendroglial Proliferation and Differentiation During Postnatal Brain Myelination and CNS Remyelination. The Journal of Neuroscience. 2018 Feb 14;38(7):1802-1820. PMID: 29335358. PMCID: PMC5815459. DOI: 10.1523/JNEUROSCI.1291-17.2018

74. Priyadarsini Kumar, Kewa Gao, Chuwang Wang, Christopher Pivetti, Lee Lankford, Diana Farmer, Aijun Wang. In Utero Transplantation of Placenta-derived Mesenchymal Stromal Cells for Potential Application in Hemophilia A Therapy. Cell Transplantation. 2018 Jan;27(1):130-139. PMID: 29562772. DOI: 10.1177/0963689717728937

Assessment of FVIII expression by transduced PMSCs
Assessment of FVIII expression by transduced PMSCs (placenta-derived mesenchymal stromal cells)
RT-PCR (A) and Western blot (B) analysis of expression of BDD-FVIII by transduced PMSCs (A: lane 2 and B: lane 2, respectively), compared to PMSCs transduced with control vector (A: lane 1 and B: lane 1, respectively). Comparison of human FVIII secretion by ELISA (C) and activity by chromogenic assay (D) between PMSCs transduced with BDD-FVIII vector and PMSCs transduced with control vector.

73. Connor Long, Lee Lankford, Priyadarsini Kumar, Robert Grahn, Dori L. Borjesson, Diana Farmer, Aijun Wang. Isolation and Characterization of Canine Placenta-derived Mesenchymal Stromal Cells for the Treatment of Neurological Disorders in Dogs. Cytometry Part A. 2018; 93(1):82-92. PMID: 28715613. DOI: 10.1002/cyto.a.23171

Co‐culture of cPMSCs with SH‐SY5Y increases neurite outgrowth
Co‐culture of cPMSCs with SH‐SY5Y increases neurite outgrowth

72. Dong Wang, LeeAnn K. Li, Tiffany Dai, Aijun Wang, Song Li. Stem Cells in Vascular Remodeling. Theranostics. 2018; 8(3):815-829. PMID: 29344309. PMCID: PMC5771096. DOI: 10.7150/thno.19577

71. Sandra Kabagambe, Benjamin Keller, James Becker, Laura Goodman, Christopher Pivetti, Lee Lankford, Karen Chung, Chelsey Lee, Y. Julia Chen, Priyadarsini Kumar, Melissa Vanover, Aijun Wang, Diana Farmer. Placental Mesenchymal Stromal Cells Seeded on Clinical Grade Extracellular Matrix Improve Ambulation in Ovine Myelomeningocele. The Journal of Pediatric Surgery. 2018, 53:178-182. PMID: 29122293. DOI: https://doi.org/10.1016/j.jpedsurg.2017.10.032

70. Y. Julia Chen, Karen Chung, Christopher Pivetti, Lee Lankford, Sandra Kabagambe, Melissa Vanover, James Becker, Chelsey Lee, Josephine Tsang, Aijun Wang, Diana Farmer. Fetal Surgical Repair with Placenta-derived Mesenchymal Stromal Cell Engineered Patch in a Rodent Model of Myelomeningocele. The Journal of Pediatric Surgery. 2018, 53:183-188. PMID: 29096888. DOI: https://doi.org/10.1016/j.jpedsurg.2017.10.040

rat MMC
Fetal surgical repair with placenta-derived mesenchymal stromal cell engineered patch in a rodent model of myelomeningocele
Operative images of the 6mm extracellular matrix patch sutured over the myelomeningocele defect on the rodent fetus; arrows correspond to suture sites (A). The patch remains in
place after the closure of the hysterotomy site (arrow) during the initial surgery (B) and at the terminal laparotomy on embryonic age (EA) 21 (C). Representative images of the
myelomeningocele defect under the operating microscope (D, E). GFP-tagged PMSC seeding onto the extracellular matrix is confirmed prior to use (F). PMSCs can be visualized on the
matrix on EA21 (G) indicating their ability to survive on the matrix for the remaining duration of gestation

69. Ting-Ya Chang, Chen Chen, Ya-Chu Chang, Chi-Huan Lu, Min Lee, Shao-Tzu Lu, De-Yao Wang, Aijun Wang, Chin-Lin Guo, Pei-Lin Cheng. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. eLife. 2017; 6:e31101. PMID:29271742. PMCID: PMC5768420. DOI: 10.7554/eLife.31101

68. Ching-Wen Huang, Wen-Chin Huang, Xuefeng Qiu, Flavia Fernandes Ferreira da Silva, Aijun Wang, Shyam Patel, Leon J. Nesti, Mu-Ming Poo, Song Li. The Differentiation Stage of Transplanted Stem Cells Modulates Nerve Regeneration. Scientific Reports. 2017; 7(1):17401. PMID: 29234013. PMCID: PMC5727226. DOI: 10.1038/s41598-017-17043-4

67. Sandra Kabagambe, Lee Lankford, Priyadarsini Kumar, Y. Julia Chen, Kyle Herout, Chelsey Lee, Rebecca Stark, Diana Farmer, Aijun Wang. Isolation of a Myogenic Progenitor Cell Population from Human Placenta: a pilot study. The Journal of Pediatric Surgery. 2017; 52(12):2078-2082. PMID: 28964407. DOI: 10.1016/j.jpedsurg.2017.08.025

Alignment of cells seeded on microfibrous scaffold
Alignment of cells seeded on microfibrous scaffold
Myogenic cells were seeded on poly (ε-caprolactone) and poly (L-lactic acid) microfibrous scaffold.
After 5 days, the cells were fused and aligned in the direction of the scaffold fibers. They were positive for myogenic markers desmin and MHC by immunocytochemistry.

66. Melissa Vanover, Aijun Wang, Diana Farmer. Potential clinical applications of placental stem cells for use in fetal therapy of birth defects. Placenta. 2017, 59:107-112. PMID: 28651900. DOI: 10.1016/j.placenta.2017.05.010

65. Y. Julia Chen, Lee Lankford, Sandra Kabagambe, Zoe Saenz, Priyadarsini Kumar, Diana Farmer, Aijun Wang. Effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells on extracellular matrix. Placenta. 2017 Apr 2. pii: S0143-4004(17)30222-9. PMID: 28465002. DOI: 10.1016/j.placenta.2017.03.022

64. Ke Cao, Jingjing Li, Jia Chen, Li Qian, Aijun Wang, Xiang Chen, Wei Xiong, jingtian tang, Shijie Tang, Yong Chen, Yao Chen, Yan Cheng, Jianda Zhou. microRNA-33a-5p increases radiosensitivity by modulating glycolysis in melanoma cells. Oncotarget. 2017; 8:83660-83672. DOI: 10.18632/oncotarget.19014

63. Dong Wang, Fan Wu, Haoyong Yuan, Aijun Wang, Gyeong Jin Kang, Tan Truong, Lu Chen, Andrew S. McCallion, Xiaohua Gong, Song Li. Sox10+ cells contribute to vascular development in multiple organs. Arteriosclerosis, Thrombosis, and Vascular Biology. 2017 Sep;37(9):1727-1731. PMID: 28751573 PMCID: PMC5572822. DOI: https://doi.org/10.1161/ATVBAHA.117.309774

62. Junlin Liao, Jia Chen, Bin He, Yong Chen, Jiaqun Xu, Hongju Xie, Feng Hu, Aijun Wang, Chengqun Luo, Qingfeng Li, Jianda Zhou. Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane. The Journal of Craniofacial Surgery. 2017; 28(6):1418-1424. PMID: 28692513. DOI: 10.1097/SCS.0000000000003739

61. Jeffrey Henry, Jian Yu, Aijun Wang, Randall Lee, Jun Fang, Song Li. Engineering the mechanical and biological properties of nanofibrous vascular grafts for in situ vascular tissue engineering. Biofabrication. 2017; 9(3):035007. PMID: 28817384. PMCID: PMC5847368. DOI: 10.1088/1758-5090/aa834b

60. Lee Lankford, Y. Julia Chen, Zoe Saenz, Priyadarsini Kumar, Connor Long, Diana Farmer, Aijun Wang. Manufacture and preparation of human placenta-derived mesenchymal stromal cells for local tissue delivery. Cytotherapy. 2017 Jun; 19(6):680-688. PMID: 28438482. DOI: 10.1016/j.jcyt.2017.03.003

2017 Cyto
PMSC manufacturing process
Summary of the PMSC manufacturing process including generation of MCB andWCB, qualification screening processes, and cell bank stability testing at 6 mo.

59. Dake Hao, Wenwu Xiao, Ruiwu Liu, Priyadarsini Kumar, Yuanpei Li, Ping Zhou, Fuzheng Guo, Diana L Farmer, Kit S. Lam, Fengshan Wang, Aijun Wang. Discovery and characterization of a potent and specific peptide ligand targeting endothelial progenitor cells and endothelial cells for tissue regeneration. ACS Chem. Biol. 2017, 12 (4), 1075–1086. PMID: 28195700. DOI: 10.1021/acschembio.7b00118

2017 ACS
Discovery and Characterization of a Potent and Specific Peptide Ligand Targeting Endothelial Progenitor Cells and Endothelial Cells for Tissue Regeneration

58. Aja Harding, Elizabeth Cortez-Toledo, Nataly Magner, Julie Beegle, Dane Coleal-Bergum, Dake Hao, Aijun Wang, Jan Nolta, Ping Zhou. Highly Efficient Differentiation of Endothelial Cells from Pluripotent Stem Cells Requires the MAPK and the PI3K Pathways. Stem Cells. 2017 Apr;35(4):909-919. PMID: 28248004. DOI: 10.1002/stem.2577

57. Dong Wang/Aijun Wang (Co-first author), Fan Wu, Xuefeng Qiu, Ye Li, Julia Chu, WC Huang, Kang Xu, Xiaohua Gong, Song Li. Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization. Scientific Reports. 2017 Jan 10;7:40295. PMID: 28071739. PMCID: PMC5223127. DOI:  10.1038/srep40295

2017 Sci Rep
Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization
GFP+ cells were immunostained with the antibodies against CD31 (a), NG2 (b) and MYH11 (c). (d) Two-photon image of mouse dorsal skinfold chamber transplanted with GFP+/Sox10+ stem cells. Dextran-Rhodamine was injected into the mouse through tail vein before imaging. Cell nuclei were stained by DAPI. Arrow, GFP+ cells. Arrowhead, double positive cells. 

2016

56. Erin G. Brown, Benjamin A. Keller, Lee Lankford, Christopher D. Pivetti, Shin Hirose, Diana L. Farmer, Aijun Wang. Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in Utero Repair of Myelomeningocele Using a Lamb Model. Fetal Diagnosis and Therapy. 2016;39(3):179-85. PMID: 26159889. DOI: 10.1159/000433427

2016 Fetal Diag
Placenta-Derived Stromal Cells for in Utero Repair of Myelomeningocele Using a Lamb Model
Cross-sectional images of the MMC lesion epicenters. Scale bar = 2 mm. A Skin-only repair. B FM only. C FM with term PMSCs. D FM with early PMSCs.

2015

55. Lin Chen, Qiang Ao, Hari Shanker Sharma, Aijun Wang, Shiqing Feng. Editorial: Neurorestoratologic Strategies and Mechanisms in the Nervous System. BioMed Research International. 2015:163170. PMID: 26491655. PMCID: PMC4603317 DOI: 10.1155/2015/163170

54. Mei Yu, Zhi-Jun Sun, Lian-Tao Li, Hong-You Ge, Chang-Qin Song, Aijun Wang. The beneficial effects of the herbal medicine Di-huang-yin-zi (DHYZ) on patients with ischemic stroke: A randomized, placebo controlled clinical study. Complementary Therapies in Medicine, 2015; 23(4): 591–597. PMID: 26275652. DOI: 10.1016/j.ctim.2015.06.003

53. Aijun Wang#, Erin G. Brown, Lee Lankford, Benjamin A. Keller, Christopher D. Pivetti, Nicole A. Sitkin, Michael S. Beattie, Jacqueline C. Bresnahan, Diana L. Farmer. Placental Mesenchymal Stromal Cells Rescue Ambulation in Ovine Myelomeningocele. Stem Cells Translational Medicine. 2015 Jun; 4(6):659-69. #Corresponding author. PMID: 25911465. PMCID: PMC4449103 DOI: 10.5966/sctm.2014-0296

2015 PMSC SCTM
PMSC treatment improves locomotor outcome
Lambs were scored using SLR. All normal lambs (n = 3) scored 15 on the SLR scale, indicating normal motor function. Vehicle‐treated MMC lambs scored from 2 to 8 on the SLR scale (2, 2, 4, 4, 6, and 8). Vehicle plus PMSC‐treated lambs scored from 5 to 15 on the SLR scale (5, 8, 10, 13, 15, and 15). Lambs in the vehicle plus PMSC treated group had significantly higher SLR scores than did the vehicle‐only lambs (p = .0108) (A). Photographs of the twin lambs are shown. A lamb treated with vehicle only was unable to bear weight on its hind limbs and is pictured with the fully extended legs typical of lambs with lower extremity paralysis (B). Its twin was treated with vehicle plus PMSCs and is shown standing independently (C). Abbreviations: MMC, myelomeningocele; PMSC, placenta‐derived mesenchymal stromal cell; SLR, sheep locomotor rating.

52. Erin G. Brown, Benjamin A. Keller, Christopher D. Pivetti, Nicole A. Sitkin, Aijun Wang, Diana L. Farmer, Jacqueline C. Bresnahan. Development of a Locomotor Rating Scale for Testing Motor Function in Sheep. The Journal of Pediatric Surgery. 2015 Apr;50(4):617-21. PMID: 25840074. DOI: 10.1016/j.jpedsurg.2015.01.002

51. Jason A. Meierhenry, Volodymyr Ryzhuk, Maricel G. Miguelino, Lee Lankford, Jerry S. Powell, Diana Farmer, Aijun Wang. Placenta as a Source of Stem Cells for Regenerative Medicine. Current Pathobiology Reports. 2015, March; 3(1): 9-16. DOI: 10.1007/s40139-015-0070-6

50. Lee Lankford, Taryn Selby, James Becker, Volodymyr Ryzhuk, Connor Long, Diana Farmer, Aijun Wang. Early Gestation Chorionic Villi-derived Stromal Cells for Fetal Tissue Engineering. World Journal of Stem Cells. 2015 Jan; 7(1): 195-207. PMCID: PMC4300931. DOI: 10.4252/wjsc.v7.i1.195

2015 WJSC
Placenta-derived mesenchymal stromal cells (PMSCs) express typical mesenchymal stromal cells surface markers and are multipotent. 
A, B: Placenta-derived mesenchymal stromal cells (PMSCs) were found to be mostly positive for well-established MSC markers CD29, CD44, CD73, CD90, and CD105, and mostly negative for hematopoietic and endothelial cell markers CD31, CD34, and CD45 (n = 6); C: Cells were multipotent and were capable of differentiating into adipocytes, osteocytes and chondrocytes in vitro. Images in the left column show negative control samples (normal media only) while those on the right display cultures grown in induction media. Yellow boxes provide a magnified view of the selected area.

2014

49. Ping Xiang, Kun-Chao Wu, Ying Zhu, Lue Xiang, Chong Li, Deng-Long Chen, Feng Chen, Guotong Xu, Aijun Wang, Min Li, Zi-Bing Jin. A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells. Biomaterials 2014 Dec;35(37):9777-88. PMID: 25220295. DOI: 10.1016/j.biomaterials.2014.08.040

48. Yujun Wei, Kai Gong, Qiang Ao, Aijun Wang, Yandao Gong, Huancong Zuo, Yuqi Zhang, James Wang, Guihuai Wang. Lentiviral vectors enveloped with rabies virus glycoprotein can be used as a novel retrograde tracer to assess nerve recovery in rat sciatic nerve injury models. Cell and Tissue Research. 2014 Feb; 355(2):255-66. PMID: 24326614. DOI: 10.1007/s00441-013-1756-x

47. Erin Brown, Payam Saadai, Christopher Pivetti, Michael Beattie, Jacqueline Bresnahan, Aijun Wang, Diana Farmer. In utero repair of myelomeningocele with autologous amniotic membrane in the fetal lamb model. The Journal of Pediatric Surgery. 2014 Jan; 49(1):133-7; discussion 137-8. PMID: 24439597. DOI: 10.1016/j.jpedsurg.2013.09.043

2014 AM Patch
Gross pathology images of the MMC lesion site after cesarean birth at GA-133 for skin only-repaired (A) and AM-repaired lambs (B). AM-repaired lambs show an inability of the skin to heal over the lesion site.

2013

46. Wei Xu, Yequan Wang, Erfu Liu, Yanjun Sun, Ziwei Luo, Zhiling Xu, Wanqian Liu, Li Zhong, Yonggang Lv, Aijun Wang, Zhenyu Tang, Song Li, Li Yang. Human iPSC-derived Neural Crest Stem Cells Promote Tendon Repair in a Rat Patellar Tendon Window Defect Model. Tissue Eng Part A. 2013 Jul; 19(21-22): 2439-2451. PMID: 23815150. PMCID: PMC3807699. DOI: 10.1089/ten.TEA.2012.0453

45. Benjamin Lee, Zhenyu Tang, Aijun Wang, Fang Huang, Zhiqiang Yan, Dong Wang, Julia Chu, Li Yang, Song Li. Synovial stem cells and their responses to the porosity of microfibrous scaffold. Acta Biomaterialia. 2013 Jul; 9(7):7264-75. PMID: 23523935. PMCID: PMC3669641. DOI: 10.1016/j.actbio.2013.03.009

44. Payam Saadai, Aijun Wang#, Yvette Nout, Timothy Downing, Michael Beattie, Jacqueline Bresnahan, Song Li, Diana Farmer. Human Induced Pluripotent Stem Cell-derived Neural Crest Stem Cells Integrate into the Injured Spinal Cord in the Fetal Lamb Model of Myelomeningocele. The Journal of Pediatric Surgery. 2013 Jan; 48(1):158-63. #Corresponding author. PMID: 23331809. DOI: 10.1016/j.jpedsurg.2012.10.034

2013 JPS NCSC
Surgical placement of the nanofibrous scaffold seeded with
human iPSC-derived NCSCs at the time of fetal lamb MMC repair.

43. Zhenyu Tang, Aijun Wang, Dong Wang, Song Li. Vascular Smooth Muscle Cells: To Be or Not To Be? Circulation Research. 2013 Jan;112(1):23-6. PMID: 23093574. DOI: 10.1161/CIRCRESAHA.112.281055

2013 Cir Res
Lineage tracing of MVSCs ex vivo and in vivo

2012

42. Jian Yu/Aijun Wang (Co-first author), Zhenyu Tang, Yiqian Zhu, Jeffrey Henry, Fengping Huang, Song Li. The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration. Biomaterials. 2012 Nov; 33(32):8062-74. PMID: 22884813. PMCID: PMC3488434. DOI: 10.1016/j.biomaterials.2012.07.042

2012 SDF
Long-term remodeling of the biodegradable vascular grafts after 6 months of implantation
(A) H&E staining of the cross-sections in the middle portion of the grafts. Immunostaining of the region in the square is shown in B and C. (B–G) The graft remodeling was characterized by immunostaining for EC marker CD31, SMC marker MHC and ECM protein elastin. Arrow in C indicates the cells infiltrated into the region of polymer degradation. (H–I) Neovascularization and tissue integration on the outer surface of the grafts were characterized by staining for CD31 and MHC. 

41. Zhenyu Tang/Aijun Wang (Co-first author), Zhiqiang Yan, Bo Liu, Julia Chu, Jill Helms, Song Li. Differentiation of Multipotent Vascular Stem Cells Contributes to Vascular Diseases. Nature Communications. 2012 Jun 6; 3:875. (Highlighted in Nature, 486, 9 (07 June 2012) DOI:10.1038/486009d.) PMID: 22673902. PMCID: PMC3538044. DOI: 10.1038/ncomms1867

Press release: This study has been reported by over 100 major news outlets worldwide.

Examples: Berkeley News, NPR’s Science Friday, San Francisco Business Times, SF Chronicle (FRONT page on 6/6/2012), Fox News, Medical News Today, US News & World Report, Daily Mail (UK).

2012 Nature Comm
Schematic illustration of the spontaneous differentiation of MVSCs and the differentiation potential of the cells at different stages.

40. Timothy Downing/Aijun Wang (Co-first author), Zhiqiang Yan, Yvette Nout, Andy Lee, Michael S. Beattie, Jacqueline C. Bresnahan, Diana L. Farmer, Song Li. Drug-eluting microfibrous patches for the local delivery of rolipram in spinal cord repair. Journal of Controlled Release. 2012; 161(3):910-917. PMCID: 22634093. PMCID: PMC3698241. DOI: 10.1016/j.jconrel.2012.05.034

2012 J Control
Implantation of drug-eluting microfibrous patches after SCI.
(A) Schematic of subdural implantation of drug-eluting microfibrous patches into the injured cord. (B) Macroscopic view of lesion site and patch during animal surgery. An asterisk was used to mark location of drug-eluting microfibrous patch. (C, D) Gross histology of spinal cord cross section 8 weeks post SCI. H&E staining reveals new tissue formation at the lesion site and patch’s ability to integrate into the surrounding tissues (D). (C) Less regenerated control for comparison. Here, arrows point to the implantation site for drug-eluting microfibrous patches. Red dashes outline areas of less tissue formation for comparison.

39. Benjamin Lee, Hojeong Jeon, Aijun Wang, Zhiqiang Yan, Jian Yu, Costas Grigoropoulos, Song Li. Femtosecond Laser Ablation Enhances Cell Infiltration into Three-Dimensional Electrospun Scaffolds. Acta Biomaterialia. 2012 Jul;8(7):2648-58. PMID: 22522128. PMCID: PMC3425609. DOI: 10.1016/j.actbio.2012.04.023

38. Aijun Wang, Zhenyu Tang, Xian Li, Yisu Jiang, Danielle A. Tsou, Song Li. Derivation of Smooth Muscle Cells with Neural Crest Origin from Human Induced Pluripotent Stem Cells. Cells Tissues Organs. 2012; 195:5-14. PMID: 22005509. PMCID: PMC3257812. DOI: 10.1159/000331412

2012 NCSC-SMC
Procedure to derive NCSCs from human ESCs and iPSCs. 

Before 2012

37. Steven Hsu, Julia Chu, Fanqing F. Chen, Aijun Wang, Song Li. Effects of Fluid Shear Stress on a Distinct Population of Vascular Smooth Muscle Cells. Cell and Molecular Bioengineering. 2011; 4:627-36. PMID: 22924082. PMCID: PMC3425612. DOI: 10.1007/s12195-011-0205-8

36. Payam Saadai, Yvette Nout, Jose Encinas, Aijun Wang, Timothy Downing, Michael S. Beattie, Jacqueline C. Bresnahan, Song Li, Diana L. Farmer. Prenatal Repair of Myelomeningocele with Aligned Nanofibrous Scaffolds - A Pilot Study in Sheep. The Journal of Pediatric Surgery. 2011; 46:2279-83. PMID: 22152865. DOI: 10.1016/j.jpedsurg.2011.09.014

35. Xian Li, Julia S. Chu, Aijun Wang, Yiqian Zhu, Wai Keung Chu, Li Yang, Song Li. Uniaxial Mechanical Strain Modulates the Differentiation of Neural Crest Stem Cells into Smooth Muscle Lineage on Micropatterned Surfaces. PLoS ONE. 2011; 6(10): e26029. PMID: 22016804. PMCID: PMC3189240. DOI: 10.1371/journal.pone.0026029

34. Yujun Wei, Kai Gong, Zhenghuan Zheng, Aijun Wang, Qiang Ao, Yandao Gong, Xiufang Zhang. Chitosan/silk Fibroin-based Tissue-engineered Graft Seeded with Adipose-derived Stem Cells Enhances Nerve Regeneration in a Rat Model. Journal of Materials Science: Materials in Medicine. 2011; 22(8):1947-64. PMID: 21656031. DOI: 10.1007/s10856-011-4370-z

33. Aijun Wang, Zhenyu Tang, In-Hyun Park, Yiqian Zhu, Shyam Patel, George Q. Daley, Song Li. Induced Pluripotent Stem Cells for Neural Tissue Engineering. Biomaterials. 2011, 32(22):5023-32. PMID: 21514663. PMCID: PMC3100451. DOI: 10.1016/j.biomaterials.2011.03.070

The top 5 most downloaded papers in April-June, 2011. Fig. 1J was selected as one of the 12 best biomaterials-related images published in the Biomaterials 2011 journal catalog.
Bio 11 Biomaterials iPSC_NCSC-PNR
Tissue-engineered nanofibrous nerve conduits for peripheral nerve regeneration.
Schematic outline of a tissue engineering approach by combining NCSCs and a nanofibrous nerve conduit. 

32. Jennifer S. Park, Anchi D. Tsou, Rokhaya Diop, Zhenyu Tang, Aijun Wang, Song Li. The Effect of Matrix Stiffness on the Differentiation of Mesenchymal Stem Cells in Response to TGF-β. Biomaterials. 2011, 32(16):3921-30. PMID: 21397942. PMCID: PMC3073995. DOI: 10.1016/j.biomaterials.2011.02.019

31. Yiqian Zhu/Aijun Wang (Co-first author), Shyam Patel, Kyle Kurpinski, Edward Diao, Xuan Bao, George Kwong, William L. Young, Song Li. Engineering Bi-layer Nanofibrous Conduits for Peripheral Nerve Regeneration. Tissue Engineering. 2011, 17(7):705-15. PMID: 21501089. PMCID: PMC3124110. DOI: 10.1089/ten.tec.2010.0565

2011 PNR TE
Photographs showing aligned nanofibrous peripheral nerve conduit for 2 months (A) and 12 months (B) postsurgery. The arrows indicate two ends of the nerve conduits. Representative micrographs of toluidine-blue staining showing the regenerated nerve and fibrous capsule layer in random (C) and aligned nanofibrous conduit groups (D) at 12 months. White dashed lines show the boundary of the fibrous capsule layer labeled with “*.” Scale bar=50 μm. (E) Quantification of the thickness of the fibrous capsule layer.

30. Hayley Lam, Shyam Patel, Aijun Wang, Julia Chu, Song Li. Regulation of Neural Differentiation and Axon Growth by Growth Factors and Bioactive Nanofibers. Tissue Engineering. 2010, 16(8):2641-8. PMID: 20367289. PMCID: PMC2947452. DOI: 10.1089/ten.TEA.2009.0414

29. Aijun Wang, Yiqian Zhu, Song Li. Nanofibrous Scaffolds for the Regeneration of Nervous Tissue. Journal of Medical Biomechanics. 2010, 25, Suppl, 20-21.

28. Yujun Wei, Zhenghuan Zheng, Lingling Liu, Aijun Wang, Lihai Zhang, Qiang Ao, Yandao Gong, Xiufang Zhang. Schwann-like Cells Differentiation of Rat Adipose-derived Stem Cells by Indirect Co-culture with Schwann Cells in vitro. Cell Proliferation. 2010, 43(6):606-16. PMID: 21039999. DOI: 10.1111/j.1365-2184.2010.00710.x

27 Gan Wang, Qiang Ao, Kai Gong, Aijun Wang, Yandao Gong, Xiufang Zhang. The Effect of Topology of Chitosan Biomaterials on the Differentiation and Proliferation of Neural Stem Cells. Acta Biomaterialia. 2010, 6(9), 3630-9. PMID: 20371303. DOI: 10.1016/j.actbio.2010.03.039

26. Yiqian Zhu/Aijun Wang (Co-first author), Wenqian Shen, Shyam Patel, Rong Zhang, William Young, Song Li. Nanofibrous Patches for Spinal Cord Regeneration. Advanced Functional Materials. 2010, 20(9), 1433-40. PMID: 23378825. PMCID: PMC3558949. DOI: 10.1002/adfm.200901889

25. Kyle Kurpinski, Hayley Lam, Julia Chu, Aijun Wang, Ahra Kim, Eric Tsay, Smita Agrawal, David Schaffer, Song Li. TGF- and Notch Signaling Mediate Stem Cell Differentiation into Smooth Muscle Cells. Stem Cells. 2010, 28(4), 734-42. PMID: 20146266. DOI: 10.1002/stem.319

24. Zhenhuan Zheng, Ling Zhang, Lijun Kong, Aijun Wang, Yandao Gong, Xiufang Zhang. The Behavior of MC3T3-E1 Cells on Chitosan/poly-L-lysine Composite Films: Effect of Nanotopography, Surface Chemistry, and Wettability. Journal of Biomedical Materials Research Part A. 2009, 89A(2):453-65. PMID: 18431777. DOI: 10.1002/jbm.a.31979

23. Yujun Wei, Jianli Zhou, Zhenghuan Zheng, Aijun Wang, Qiang Ao, Yandao Gong, Xiufang Zhang. An Improved Method for Isolating Schwann Cells from Postnatal Rat Sciatic Nerves. Cell and Tissue Research. 2009, 337(3):361-9. PMID: 19639342. DOI: 10.1007/s00441-009-0836-4

22. Zhenhuan Zheng, Yujun Wei, Gan Wang, Aijun Wang, Qiang Ao, Xiufang ZhangSurface Properties of Chitosan Films Modified with Polycations and Their Effects on the Behavior of PC12 Cells. Journal of Bioactive and Compatible Polymers. 2009, 24(1): 63-82. DOI: 10.1177/0883911508099653

21. Fangfang Zhou, Long Zhang, Kai Gong, Guangyuan Lu, Baiyang Sheng, Aijun Wang, Nanming Zhao, Xiufang Zhang, Yandao Gong. LEF-1 Activates the Transcription of E2F1. Biochemical and Biophysical Research Communications. 2008, 365(1):149-53. PMID:17980157. DOI: 10.1016/j.bbrc.2007.10.138

20. Fangfang Zhou, Long Zhang, Aijun Wang, Bo Song, Kai Gong, Lihai Zhang, Min Hu, Xiufang Zhang, Nanming Zhao, Yandao Gong. The association of GSK3β with E2F1 Facilitates NGF-induced Neural Cell Differentiation. The Journal of Biological Chemistry 2008, 283(21):14506-15. PMID: 18367454. DOI: 10.1074/jbc.M706136200

19. Qiang Ao, Aijun Wang, Zhigang Sun, Xiufang Zhang. Preparation and Characterization of a Chitosan Conduit for Neural Tissue Engineering. Journal of Clinical Rehabilitative Tissue Engineering Research. 2008,12(1):1-5. (in Chinese)

18. Lijun Kong, Qiang Ao, Aijun Wang, Kai Gong, Xi Wang, Guangyuan Lu, Yandao Gong, Nanming Zhao, Xiufang Zhang. Preparation and Characterization of a Multilayer Biomimetic Scaffold for Bone Tissue Engineering. Journal of Biomaterials Applications. 2007, 22(3):223-39. PMID: 17255157. DOI: 10.1177/0885328206073706

17. Qiang Ao/Aijun Wang (Co-first author), Guoqiang Chen, Shijie Wang, Huancong Zuo, Xiufang Zhang. Combined Transplantation of Neural Stem Cells and Olfactory Ensheathing Cells for the Repair of Spinal Cord Injuries. Medical Hypotheses. 2007, 69(6):1234-7. PMID: 17548168. DOI: 10.1016/j.mehy.2007.04.011

16. Aijun Wang, Qiang Ao, Yujun Wei, Kai Gong, Nanming Zhao, Yandao Gong, Xiufang Zhang. Physical Properties and Biocompatibility of a Porous Chitosan-based Fibre-reinforced Conduit for Nerve Regeneration. Biotechnology Letters. 2007, 29(11):1697-702. PMID: 17628751. DOI: 10.1007/s10529-007-9460-0

This article is in the 61st percentile (ranked 50,481st) of the 131,642 tracked articles of a similar age in all journals and the 91st percentile (ranked 1st) of the 12 tracked articles of a similar age in Biotechnology Letters

15. Qing He, Qiang Ao, Aijun Wang, Yandao Gong, Nanming Zhao, Xiufang Zhang. In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Microspheres. Tsinghua Science and Technology. 2007, 12(4): 361-5. DOI: 10.1016/S1007-0214(07)70054-8http://dx.doi.org/10.1016/S1007-0214(07)70054-8

14. Lijun Kong, Qiang Ao, Aijun Wang, Kai Gong, Xi Wang, Yandao Gong, Nanming Zhao, Xiufang Zhang. Effect of Multilayer Biomimetic Nano-hydroxyapatite/chitosan Composite Scaffolds on Repairing Rabbit Fibula Defect. Journal of Clinical Rehabilitative Tissue Engineering Research. 2007,11(5):815-8. (in Chinese). https://www.researchgate.net/publication/289869069_Effect_of_multilayer_biomimetic_nano-hydroxyapatitechitosan_composite_scaffolds_on_repairing_rabbit_fibula_defect

13. Qiang Ao, Xiuhua Sun, Aijun Wang, Panfeng Fu, Kai Gong, Yandao Gong, Xiufang Zhang. Protective Effects of Extract of Ginkgo Biloba (EGb 761) on Nerve Cells after Spinal Cord Injury in Rats. Spinal Cord. 2006, 44(11): 662-7. PMID: 16415923. DOI: 10.1038/sj.sc.3101900

12. Aijun Wang, Qiang Ao, Kai Gong, Zhenhuan Zheng, Guangyuan Lu, Gan Wang, Qing He, Lijun Kong, Yandao Gong, Nanming Zhao, Xiufang Zhang. Chitosan-based Semi-permeable Nerve Conduits Support Peripheral Nerve Regeneration in Goats and Nonhuman Primates. Molecular and Cellular Biomechanics. 2006, 3 (4): 143-4.

11. Hongxu Qi, Ping Hu, Jun Xu, Aijun Wang. Encapsulation of Drug Reservoirs in Fibers by Emulsion Electrospinning: Morphology Characterization and Preliminary Release Assessment. Biomacromolecules. 2006, 7(8): 2327-30. PMID: 16903678. DOI: 10.1021/bm060264z

2004 Biomacro
Encapsulation of Drug Reservoirs in Fibers by Emulsion Electrospinning

10. Ling Zhang, Qiang Ao, Aijun Wang, Guangyuan Lu, Lijun Kong, Yandao Gong, Nanming Zhao, Xiufang Zhang. A Sandwich Tubular Scaffold Derived from Chitosan for Blood Vessel Tissue Engineering. Journal of Biomedical Materials Research Part A. 2006, 77A(2):277-84. PMID: 16400655. DOI: 10.1002/jbm.a.30614

9. Qiang Ao, Aijun Wang, Wenling Cao, Ling Zhang, Lijun Kong, Qing He, Yandao Gong, Xiufang Zhang. Manufacture of Multi-microtubule Chitosan Nerve Conduits with Novel Molds and Characterization in vitro. Journal of Biomedical Materials Research Part A. 2006, 77A(1):11-8. PMID: 16345091. DOI: 10.1002/jbm.a.30593

8. Aijun Wang, Wenling Cao, Kai Gong, Qiang Ao, Lijun Kong, Chengzhao He, Yaodao Gong, Xiufang Zhang. Development of Porous Chitosan Tubular Scaffolds for Tissue Engineering Applications. Asian Chitin Journal. 2006, 2: 53-60.

7. Aijun Wang, Wenling Cao, Qiang Ao, Kai Gong, Lijun Kong, Yaodao Gong, Xiufang Zhang. Controlling Morphology and Porosity of 3-D Chitosan Scaffolds Produced by Thermally Induced Phase Separation Technique. Asian Chitin Journal. 2006, 2: 69-78.

6. Aijun Wang, Qiang Ao, Wenling Cao, Mingzhi Yu, Qing He, Lijun Kong, Ling Zhang, Yandao Gong, Xiufang Zhang. Porous Chitosan Tubular Scaffolds with Knitted Outer Wall and Controllable Inner Structure for Nerve Tissue Engineering. Journal of Biomedical Materials Research Part A. 2006, 79A(1): 36-46. PMID: 16758450. DOI: 10.1002/jbm.a.30683

2006 JBMR
Scanning electron micrographs of transverse cross-sections of the guidance scaffolds
(A) the knitted outer tube wall with fiber‐based structure, (B) the highly porous inner matrices with axially oriented macrochannels and many interconnected micropores

5. Aijun Wang, Qiang Ao, Qing He, Xiaoming Gong, Kai Gong, Yandao Gong, Nanming Zhao, Xiufang Zhang. Neural Stem Cell Affinity of Chitosan and Feasibility of Chitosan-based Porous Conduits as Scaffolds for Nerve Tissue Engineering. Tsinghua Science and Technology. 2006, 11(4):415-20. http://dx.doi.org/10.1016/S1007-0214(06)70210-3

4. Aijun Wang, Qiang Ao, Wenling Cao, Chang Zhao, Yandao Gong, Nanming Zhao, Xiufang Zhang. Fiber-based Chitosan Tubular Scaffolds for Soft Tissue Engineering: Fabrication and in vitro Evaluation. Tsinghua Science and Technology. 2005, 10(4):449-53. DOI: 10.1016/S1007-0214(05)70099-7http://dx.doi.org/10.1016/S1007-0214(05)70099-7

3. Wenling Cao, Aijun Wang, Duohui Jing, Yandao Gong, Nanming Zhao, and Xiufang Zhang. Novel Biodegradable Films and Scaffolds of Chitosan Blended with Poly(3-hydroxybutyrate). Journal of Biomaterials Science, Polymer Edition. 2005, 16(11):1379-94. PMID: 16370239. DOI: 10.1163/156856205774472308

2. Qiang Ao, Aijun Wang, Wenling Cao, Chang Zhao, Yandao Gong, Nanming Zhao, Xiufang Zhang. Fabrication and Characterization of Chitosan Nerve Conduits with Microtubular Architecture. Tsinghua Science and Technology. 2005, 10(4):435-8. DOI: 10.1016/S1007-0214(05)70096-1

1. Qiang Ao, Aijun Wang, Wengling Cao, Chang Zhao, Yandao Gong, Nanming Zhao, Xiufang Zhang. Preparation of Porous Multi-channeled Chitosan Conduits for Nerve Tissue Engineering. Key Engineering Materials. 2005, (288-289): 27-30. DOI: 10.4028/www.scientific.net/KEM.288-289.27
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